Purification of the Infectious Bovine Rhinotracheitis Virus Alkaline Deoxyribonuclease Expressed in Escherichia coli.

The Isolation and Identification of Infectious Bovine Rhinotracheitis Virus in Iran

PURIFICATION AND CHARACTERIZATION OF THE CLONED HUMAN GM-CSF GENE EXPRESSED IN ESCHERICHIA COLI

The human granulocyte-macrophage colony stimulation factor (hGM-CSF) gene was cloned in the pET 23a( +) expression vector under the control of strong bacteriophage T7 transcription and translation signals. The hGM-CSF gene was transferred into E. coli strainBL21 (DE3)pLysS andIPTG was used for induction of GM-CSF gene. Production of the target protein was obtained as revealed by ELISA and ...

Susceptibility of bovine macrophages to infectious bovine rhinotracheitis virus infection.

Infectious bovine rhinotracheitis virus replicated in cultured bovine alveolar macrophages (AM). However, yields of infectious virus were low, with maximum titers approximately 100 times that of the residual inoculum. Immunofluorescence and electron microscopic studies indicated that the majority of macrophages produced viral antigen, but after infection at a multiplicity of 0.1, only 4.1% of A...

Infectious bovine rhinotracheitis.

Cattle exhibiting the vulvovaginitis form of the IBR complex are sexually mature females that do not appear ill. Signs of IPV include a thick yellow to brown vulvar discharge that attaches to the vulvar tuft of hair. The vulva is swollen and the vulvar and vaginal lining is reddened, dying and/or contains small whitish-colored pustules. The vaginal-vulvar infection causes irritation, exhibited ...

Sensitivity of infectious bovine rhinotracheitis virus to ether.

The sensitivity of 12 field isolates of infectious boviine rhinotracheitis (IBR) virus and four commercial modified-live infectious bovine rhinotracheitis virus vaccine strains was determined after exposure to 20% ethyl ether (anesthetic) for 16 h at 4 c. The infectivity of five field strains was reduced by varying degrees, whereas seven were found to be resistant. Three vaccine strains were mo...